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Comparing cDNA and Oligonucleotide Array Data: Concordance of Gene
Expression Across Platforms for the NCI-60 Cancer Cells
Lee JK, Bussey KJ, Gwadry FG, Reinhold W, Riddick G,
Pelletier SL, Nishizuka S, Szakacs G, Annereau JP, Shankavaram U, Lababidi S,
Smith LH, Gottesman MM, Weinstein JN.
Genome Biol. 2003;4(12):R82
Abstract:
Microarray gene-expression profiles are generally validated one gene at a time by
real-time RT-PCR. We describe here a different approach based on simultaneous mutual
validation of large numbers of genes using two different expression-profiling platforms.
The result described here for the NCI-60 cancer cell lines is a consensus set of genes
that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips.
Global concordance is parameterized by a 'correlation of correlations' coefficient.
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